Ginkgetin, a biflavone from Ginkgo biloba leaves, was ahead appear to be a phospholipase A(2) inhibitor and this admixture showed the almighty antiarthritic action in rat adjuvant-induced arthritis as able-bodied as analgesic activity. This analysis was agitated out to acquisition furnishings on cyclooxygenase-2 (COX-2) in vitro effect.
Ginkgetin inhibits COX-2 abased phases of prostaglandin D(2) (PGD(2)) bearing in cartilage marrow-derived mast beef (BMMC) in a concentration-dependent address with IC(50) ethics of 0.75 microM. Western blotting probed with specific anti-COX-2 antibodies showed that the abatement in abundance of the PGD(2) artefact was accompanied by a abatement in the COX-2 protein level. In addition, this admixture consistently inhibited the assembly of leukotriene C(4) (LTC(4)) in a dosage abased manner, with an IC(50) amount of 0.33 microM.
These after-effects authenticate that ginkgetin has a bifold cyclooxygenase-2/5-lipoxygenase inhibitory activity. Furthermore, this admixture aswell inhibited degranulation acknowledgment in a dosage abased manner, with an IC(50) amount of 6.52 microM. ThereforGinkgetinGinkgetine, this admixture ability accommodate a base for atypical anti-inflammatory agents.
